| Autor: |
Amram, E., Mikula, I., Schnee, C., Ayling, R. D., Nicholas, R. A. J., Rosales, R. S., Harrus, S., Lysnyansky, I. |
| Zdroj: |
Antimicrobial Agents and Chemotherapy; November 2014, Vol. 59 Issue: 2 p796-802, 7p |
| Abstrakt: |
ABSTRACTMycoplasma bovisisolates with decreased susceptibilities to tetracyclines are increasingly reported worldwide. The acquired molecular mechanisms associated with this phenomenon were investigated in 70 clinical isolates of M. bovis. Sequence analysis of the two 16S rRNA-encoding genes (rrs3and rrs4alleles) containing the primary binding pocket for tetracycline (Tet-1 site) was performed on isolates with tetracycline hydrochloride MICs of 0.125 to 16 μg/ml. Mutations at positions A965T, A967T/C (Escherichia colinumbering) of helix 31, U1199C of helix 34, and G1058A/C were identified. Decreased susceptibilities to tetracycline (MICs, ≥2 μg/ml) were associated with mutations present at two (A965 and A967) or three positions (A965, A967, and G1058) of the two rrsalleles. No tet(M), tet(O), or tet(L) determinants were found in the genome of any of the 70 M. bovisisolates. The data presented correlate (P< 0.0001) the mutations identified in the Tet-1 site of clinical isolates of M. boviswith decreased susceptibility to tetracycline. |
| Databáze: |
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