Autor: |
Soltys, Daniela Tathiana, Pereira, Carolina Parga Martins, Ishibe, Gabriela Naomi, de Souza-Pinto, Nadja Cristhina |
Zdroj: |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis; June 2015, Vol. 776 Issue: 1 p48-53, 6p |
Abstrakt: |
Most human tissues used in research are of post mortemorigin. This is the case for all brain samples, and due to the difficulty in obtaining a good number of samples, especially in the case of neurodegenerative diseases, male and female samples are often included in the same experimental group. However, the effects of post morteminterval (PMI) and gender differences in the endpoints being analyzed are not always fully understood, as is the case for DNA repair activities. To investigate these effects, in a controlled genetic background, base excision repair (BER) activities were measured in protein extracts obtained from Wistar rat brains from different genders and defined PMI up to 24hours, using a novel fluorescent-based in vitroincision assay. Uracil and AP-site incision activity in nuclear and mitochondrial extracts were similar in all groups included in this study. Our results show that gender and PMI up to 24hours have no influence in the activities of the BER proteins UDG and APE1 in rat brains. These findings demonstrate that these variables do not interfere on the BER activities included in these study, and provide a security window to work with UDG and APE1 proteins in samples of post mortemorigin. |
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