Molecular Mapping of a Stripe Rust Resistance Gene YrH9020aTransferred from Psathyrostachys huashanicaKeng on Wheat Chromosome 6D

Autor: LIU, Ze-guang, YAO, Wei-yuan, SHEN, Xue-xue, CHAO, Kai-xiang, FAN, Yu, LI, Min-zhou, Bao-tong, WANG, LI, Qiang, JING, Jin-xue
Zdroj: Journal of Integrative Agriculture; December 2014, Vol. 13 Issue: 12 p2577-2583, 7p
Abstrakt: Stripe rust (yellow rust), caused by Puccinia striiformisf. sp. tritici(Pst), is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspecific hybridization between wheat line 7182 and Psathyrostachys huashanicaKeng and is resistant to most Pstraces in China. To identify the resistance gene(s) in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1generations were tested with prevalent Chinese Pstrace CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a,conferring resistance to CYR32. The resistance gene was mapped by the F2population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21,and Xgdm141,flanked by Xbarc96and Xbarc202with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring (CS) nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020amight be a new resistance gene to stripe rust. The flanking markers linked to YrH9020acould be useful for marker-assisted selection in breeding programs.
Databáze: Supplemental Index