| Autor: |
McGeown, J G, Drummond, R M, McCarron, J G, Fay, F S |
| Zdroj: |
Journal of Physiology; December 1996, Vol. 497 Issue: 2 p321-336, 16p |
| Abstrakt: |
1. Decay in intracellular calcium concentration ([Ca2+]i) was recorded following step depolarizations in voltage clamped gastric myocytes from Bufo marinus. 2. Depolarizations (300 ms) to +10 mV were followed by three phases of [Ca2+]i decay with repolarization to both ‐110 and ‐50 mV. The decline was initially rapid (mean fractional decay rate = 81 +/‐ 11%s‐1 at ‐110 mV), then slowed (decay rate = 14 +/‐ 2%s‐1) and finally accelerated again (decay rate = 24 +/‐ 3%s‐1; n = 19). 3. The initial phase of rapid decay became shorter as the length of the depolarizing pulse increased but was unaffected by changes in pulse voltage. 4. The delayed acceleration in [Ca2+]i decay was no longer seen when the duration of the depolarizing pulses was reduced to 100 ms, but was clearly evident following 500 ms pulses. This phase was abolished when the depolarizing voltage was altered to minimize the rise in [Ca2+]i. 5. Ryanodine and caffeine had no effect on the temporal profile of [Ca2+]i decay. 6. Removal of extracellular Na+ decreased the decay rate during all three phases at ‐110 mV, but this effect was particularly marked for the initial rapid phase of decay, the rate of which was reduced by 75%. A delayed increase in decay rate was still seen. 7. Inhibition of mitochondrial Ca2+ uptake with cyanide, carbonyl cyanide p‐trifluoromethoxy‐phenylhydrazone or Ruthenium Red had no effect on the initial rate of [Ca2+]i decay but blocked the delayed acceleration. 8. These results are discussed in terms of a model in which rapid influx of Ca2+ produces a high subsarcolemmal [Ca2+], favouring rapid Ca2+ removal by near‐membrane mechanisms, particularly Na(+)‐Ca2+ exchange. Mitochondrial Ca2+ removal produces a delayed increase in [Ca2+]i decay if the global [Ca2+]i is raised high enough for long enough. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Plný text