Active complex formation of type I and type II activin and TGFβ receptors in vivo as studied by overexpression in zebrafish embryos

Autor: de Vries, Carlie J.M., de Boer, Jan, Joore, Jos, Stra¨hle, Uwe, van Achterberg, Tanja A.E., Huylebroeck, Danny, Verschueren, Kristin, Miyazono, Kohei, van den Eijnden-van Raaij, Adriana J.M., Zivkovic, Danica
Zdroj: Mechanisms of Development; 1996, Vol. 54 Issue: 2 p225-236, 12p
Abstrakt: We have investigated the involvement of activin receptors and TGFβ type I receptor in zebrafish development. Overexpression of either full-length or a truncated form of mouse ActR-IIA interferes with the development. Different splice variants of mouse ActR-IIB have distinct effects; ActR-IIB4 induces abnormal embryos, whereas ActR-IIB2 does not. Activin and TGFβ type I receptors can induce axis duplications. Co-expression of ActR-IA or ActR-IB with the type II activin receptors results in a synergistic increase of the frequency of axis duplication. Moreover, ActR-IIB2 is synergistic with ActR-IA and ActR-IB, demonstrating that ActR-IIB2 can interact with the zebrafish ligand. Overexpression of TGFβR-I with ActR-IIA or ActR-IIB4 results in a synergistic increase in frequency of abnormal embryos, whereas in combination with ActR-IIB2 no such increase occurs.
Databáze: Supplemental Index
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