Identification of Male Bloodstains by Dot Hybridization of Human Y Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe

Autor: Fukushima, H, Hasekura, H, Nagai, K
Zdroj: Journal of Forensic Sciences; May 1988, Vol. 33 Issue: 3 p621-627, 7p
Abstrakt: The sex determination of bloodstains was performed using a human Y chromosome-specific (DNA) fragment of 1.9-kb length as a hybridization probe. The DNA samples were taken from 1- and 4-week-old bloodstains of males and females, respectively. Strong signals with male DNA were observed by Y-probe, while faint signals with female DNA were detected. In addition, clear signals were observed in the extract samples from male bloodstains (16-week-old) on paper. Dot hybridization of the Y-probe would be widely applicable to studies on sex determination of medicolegal materials such as blood, bloodstains, teeth, and cadaverous parts.
Databáze: Supplemental Index