The Use of Ultrathin-Layer Agarose Gels for Phenotyping Erythrocyte Acid Phosphatase by Isoelectric Focusing
| Autor: | Frank, WE, Stolorow, MD |
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| Zdroj: | Journal of Forensic Sciences; July 1986, Vol. 31 Issue: 3 p1089-1094, 6p |
| Abstrakt: | A method is described for the use of ultrathin-layer agarose gels in phenotyping erythrocyte acid phosphatase (EAP) by isoelectric focusing (IEF). The results obtained using ultrathin-layer agarose gels are shown to be equally reliable and reproducible in comparison to established ultrathin-layer polyacrylamide gels. IEF of EAP on 0.168-mm agarose gels took place in 90 min using the LKB Multiphor system. The technique described allows for both time and cost efficient phenotyping of EAP. |
| Databáze: | Supplemental Index |
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