| Autor: |
Pacheco, R.S., Thomaz, N., Brandão, A.A., Pires, M.Q., Momen, H., Degrave, W., Pacheco, R.S., Thomaz, N., Brandão, A.A., Pires, M.Q., Momen, H., Degrave, W. |
| Zdroj: |
Parasite - Journal de la Société Française de Parasitologie; September 1996, Vol. 3 Issue: 3 p297-299, 3p |
| Abstrakt: |
The article reports the use of synthetic oligonucleotides as an additional tool for discriminating between the subgenera Schizotrypanumand Megatrypanumof the genus Trypanosoma. Three oligonucleotides were synthezised based on conserved sequence blocks in the minirepeat of the kinetoplast DNA minicircles (kDNA| from T. cruziand used as probes in dot-blot hybridization experiments against kDNA from trypanosomatids belonging to different genera. Under specific hybridization and washing conditions the oligoprobes were found to hybridize distinctly with protozoa from the subgenera Schizotrypanumand Megatrypanumand with two monogenetic insect trypanosomatids. One of the oligonucleotides showed to be specific for the subgenus Schizotrypanum. No cross-hybridization was detected with T. rangeli, which, in fact, belongs to another subgenus (Tejeraia, Añez, 1982), confirming the specificity of the probe. This promissing result reinforces the use of oligoprobes for typing of trypanosomes. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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