| Autor: |
Feliciangeli, M.D., Rodriguez, N., De Guglielmo, Z., Rodriguez, A., Feliciangeli, M.D., Rodriguez, N., De Guglielmo, Z., Rodriguez, A. |
| Zdroj: |
Parasite - Journal de la Société Française de Parasitologie; June 1999, Vol. 6 Issue: 2 p113-120, 8p |
| Abstrakt: |
As part of an epidemiological study in an old focus of American Visceral Leishmaniasis (AVL) in Venezuela (Guayabita, Aragua State], a longitudinal entomological survey (January 1993-June 1994) was carried out. A total of 3,239 males and 6,043 females belonging to 11 phlebotomine sandfly species were collected. The two recognised vectors of AVL in the New World, Lutzomyia evansiand Lu. longipalpiswere found to be sympatric. Lutzomyia evansiwas the dominant species (86.4 %), almost ten fold times more abundant than Lu. longipalpis(10.6 %). The two species alternated seasonally: Lu evansipeaked at the end of the rainy season while Lu. longipalpis, almost virtually absent during such period, increased in the dry season. This species seems more greatly influenced by the temperature. Seven of 4,559 Lutzomyia evansi(0.15 %) and one of 353 Lu. longipalpis(0.28 %) were found positive for suprapyloric promastigotes. Using the polymerase chain reaction (PCR) with universal primers, all isolates were identified as Leishmaniaspp. Two cultures from Lu. evansi, IEVA/VE/93/UCNA-2 and IEVA/VE/93/UCNA-3, were established. k-DNA restriction analysis showed high homologies between these isolates and Leishmania chagasi. High hybridization signal with Í. chagasispecific kDNA confirmed these results. These findings suggest that Lu. evansimay play a role as vector of visceral leishmaniasis in this area. The identity of the parasite carried by Lu. longipalpisneeds to be confirmed. |
| Databáze: |
Supplemental Index |
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