| Autor: |
Moreira, Monique R., Souza, Ariana B., Moreira, Maísa A., Bianchi, Thamires C., Carneiro, Luiza J., Estrela, Fernanda T., dos Santos, Raquel A., Januário, Ana H., Martins, Carlos H.G., Ambrosio, Sérgio R., Veneziani, Rodrigo C.S. |
| Zdroj: |
Revista Brasileira de Farmacognosia; November-December 2013, Vol. 23 Issue: 6 p870-876, 7p |
| Abstrakt: |
In this paper we screened the dichloromethane extract from the aerial parts of Salvia officinalisL., Lamiaceae, against a representative panel of microorganisms that cause caries, conducted a bioassay-guided fractionation to establish themselves the most active metabolite (manool) and determined the Salvia officinalisfraction with the manool highest concentration to be used to activate an ingredient in oral care products such as toothpastes and mouthwashes. Both manool and S. officinalisextract showed very promising minimal inhibitory concentration values (between 6.24 and 31.36μg.ml−1) and time kill curves against the primary causative agents of dental caries (Streptococcus mutans) revealed that, at twice its minimal bactericidal concentration (12.48μg.ml−1), manool required 6h to completely kill the bacteria. Salvia officinalisextract at twice its minimal bactericidal concentration (31.36μg.ml−1) needed 12h. The results achieved with Salvia officinalisextract motivated us to develop and validate an analytical RP-HPLC method to detect and determine manool in this extract. The validation parameters were satisfactorily met and evaluated allows us to consider the developed method suitable for use in different labs. In conclusion, our results evidenced that the manool-rich S. officinalisextract can be considered an analytically validated alternative to develop novel and effective antimicrobial agents against the main bacteria responsible for dental caries. |
| Databáze: |
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