Autor: |
Rar, Vera A., Epikhina, Tamara I., Yakimenko, Valeriy V., Malkova, Marina G., Tancev, Aleksey K., Bondarenko, Evgeny I., Ivanov, Mikhail K., Tikunova, Nina V. |
Zdroj: |
Ticks and Tick-borne Diseases; October 2014, Vol. 5 Issue: 6 p854-863, 10p |
Abstrakt: |
Anaplasma phagocytophilumis a causative agent of granulocytic anaplasmosis in different mammals. The presence of A. phagocytophilumwas assayed in Ixodes persulcatus, Ixodes triangulicepsticks and Myodesspp. voles from two I. persulcatus/I. triangulicepssympatric areas in the Omsk region (Western Siberia, Russia). In total, A. phagocytophilumwas found in 42/108 (38.9%) of vole blood samples, 13/34 (38.2%) of I. triangulicepsticks removed from voles, 1/12 (8.3%) of I. persulcatusremoved from voles, and 18/279 (7.2%) of questing I. persulcatus. GroESLoperon sequence analysis of positive samples revealed three distinct A. phagocytophilumgenetic groups previously identified in ticks and mammals in Russia. Genetic group 1 was found in 6/36 (16.7%) of sequenced positive blood samples; this group was previously revealed in I. persulcatusand Myodesspp. voles in different regions of Russia. Genetic group 2 was found in 30/36 (83.3%) of sequenced positive blood samples and all positive I. trianguliceps; this group was previously revealed only in Myodesspp. voles and common shrews (Sorex araneus) in I. persulcatus/I. triangulicepssympatric areas in the Northern Ural. Genetic group 3 was found in all positive questing I. persulcatusand one blood sample; this group was previously revealed in I. persulcatusand Siberian chipmunks (Tamias sibiricus). We suppose that I. triangulicepsis the most probable vector for A. phagocytophilumof group 2. Analysis of the msp4gene, intergenic region DOV1, and some other genetic loci has shown that isolates from different genetic groups significantly differ in all studied loci and that A. phagocytophilumof group 2 is closely related to A. phagocytophilumisolates revealed in voles and I. triangulicepsin Europe. A. phagocytophilumof groups 1 and 2 are the most similar to each other, while A. phagocytophilumof group 3 clusters with European A. phagocytophilumisolates from I. ricinusand various mammalian species. |
Databáze: |
Supplemental Index |
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