| Autor: |
Masry, Saad Hamdy Daif, Kabeil, Sanaa Soliman, Hafez, Elsayed Elsayed |
| Zdroj: |
Biotechnology & Biotechnological Equipment; March 2014, Vol. 28 Issue: 2 p271-276, 6p |
| Abstrakt: |
The American foulbrood disease is widely distributed all over the world and causes a serious problem for the honeybee industry. Different infected larvae were collected from different apiaries, ground in phosphate saline buffer (PSB) and bacterial isolation was carried out on nutrient agar medium. Different colonies were observed and were characterized biologically. Two bacterial isolates (SH11 and SH33) were subjected to molecular identification using 16S rRNA gene and the sequence analysis revealed that the two isolates are Paenibacillus larvaewith identity not exceeding 83%. The DNA sequence alignment between the other P. larvaebacterial strains and the two identified bacterial isolates showed that all the examined bacterial strains have the same ancestor, i.e. they have the same origin. The SH33 isolate was closely related to the P. larvaeisolated from Germany, whereas the isolate SH11 was close to the P. larvaeisolated from India. The phylogenetic tree constructed for 20 different Bacillussp. and the two isolates SH11 and SH33 demonstrated that the two isolates are Bacillussp. and they are new isolates. The bacterial isolates will be subjected to more tests for more confirmations. |
| Databáze: |
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