| Autor: |
Yamada, Kiyoshi, Brink, Ingo, Engelhardt, Rupert |
| Zdroj: |
The Journal of Dermatology; May 2005, Vol. 32 Issue: 5 p335-345, 11p |
| Abstrakt: |
In order to specify the influence of multidrug‐resistance (MDR) on the accumulation of the PET tracer, F‐18 FDG ([Fluorine‐18] 2‐fluoro‐2‐deoxy‐d‐glucose, in melanoma cells, both the MDR function and expression of two human melanoma cell lines SK‐MEL 23 and 24, were evaluated. The effects of MDR modulators on FDG accumulation and efflux were also investigated. A functional analysis using representative MDR fluorescent substrates and inhibitors clarified the following characteristics: 1) SK‐MEL 23 possesses a highly active function of MRP, but not P‐gp. 2) SK‐MEL 24 possesses weak functions of both MRP and P‐gp. Western blot analysis using monoclonal antibodies for MDR expression demonstrated an exceedingly high MRP expression of SK‐MEL 23 and only slight P‐gp and MRP expression of SK‐MEL 24, corresponding to the functional data. The efflux inhibition assay using F‐18 FDG revealed a considerable retention of FDG in SK‐MEL 23 in the presence of the MRP inhibitor probenecid. It was also found that the P‐gp inhibitor verapamil depressed the FDG efflux of SK‐MEL 24. Our present in vitrostudy suggests that FDG may be a substrate of MDR in some melanoma cells and further MDR may be one of the important factors affecting FDG‐PET melanoma imaging. |
| Databáze: |
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