| Autor: |
Probert, William S., McQuaid, Cassandra, Schrader, Kimmi |
| Zdroj: |
Journal of Clinical Microbiology; July 2014, Vol. 52 Issue: 7 p2346-2351, 6p |
| Abstrakt: |
ABSTRACTWe describe here the isolation and identification of a Shiga toxin 1 (Stx1)-producing Enterobacter cloacaestrain, M12X01451, from a human clinical specimen. The bacterial isolate was identified as E. cloacaeusing a polyphasic approach that included phenotypic, genetic, and proteomic analyses. The M12X01451 stx1was sequenced, and the holotoxin was found to share only 87% amino acid sequence identity with the nearest Stx1 subtype reference sequence. Sequence analysis of the regions immediately flanking stx1displayed similarities with bacteriophage-related sequences, suggesting a prophage origin. The stx1gene was a stable element within the M12X01451 genome, as demonstrated by real-time PCR detection following successive subculturing of the bacterial isolate. Culture supernatant from M12X01451 was cytotoxic to Vero cells but was not neutralized by an anti-Stx1 monoclonal antibody. In addition, Stx1 from M12X01451 demonstrated limited antigenicity with two commercially available lateral flow immunoassays. The M12X01451 Stx represents a new Stx1 subtype based on the degree of sequence dissimilarity with Stx1 subtype reference sequences and its limited reactivity with anti-Stx1 antibodies. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
