| Autor: |
KOHASHI, Masahiro, OHTA, Yuka, WATANABE, Tatsuo |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; January 1998, Vol. 62 Issue: 2 p297-301, 5p |
| Abstrakt: |
Non-thermal effect of a ceramics radiation on glucose-6-phosphate dehydrogenase has been investigated using the enzyme, glucose-6-phosphate and NADP+separately irradiated at 10°C by a ceracompo R plate and a ceramics un-sewed cloth (sheet). The Kmfor glucose-6-phosphate was increased 20% after 6 h of irradiation by the plate, but the Vmax/Kmwas decreased 24%. After 3 h of irradiation by the sheet, the Kmwas increased 17%, but after 6 h of irradiation it was decreased 11%. The 3 h of irradiation by the sheet slightly increased both enthalpy and entropy changes of the reaction, but the 6 h of irradiation significantly decreased them. Both thermodynamic parameters in the activated state were increased by the sheet irradiation. The promotion energy for both formations of the enzyme-substrate and their activated complex depended on enthalpy. The different effects of two ceramics radiators on G6PDH activity were discussed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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