| Autor: |
INOUE, Toyohisa, IEFUJI, Haruyuki, FUJII, Tsutomu, SOGA, Hiroshi, SATOH, Kaname |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; January 2000, Vol. 64 Issue: 2 p229-236, 8p |
| Abstrakt: |
Ethanol-sensitive mutants (es1 to es10) were isolated from sakeyeast, Saccharomyces cerevisiaeSY-32. These mutants were unable to grow at 7% ethanol at which the wild type strain SY-32 does grow. The mutants had a variety of fermentation rates and viabilities in the presence of ethanol. The gene ERG6, com- plementing the ethanol-sensitive mutation of es5, was cloned from an SY-32 gene library. ERG6encodes S-adenosylmethionine: delta 24-sterol-C-methyltransferase (EC 2.1.1.41) in the ergosterol synthetic pathway. Mutant es5 had a reduced ability to synthesize ergosterol. An erg6disruptant was also ethanol-sensitive. These results suggested that ERG6plays an important role in the ethanol tolerance of S. cerevisiae. |
| Databáze: |
Supplemental Index |
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