| Autor: |
ASO, Yuji, SASHIHARA, Toshihiro, NAGAO, Jun-ichi, KANEMASA, Youhei, KOGA, Hanako, HASHIMOTO, Taku, HIGUCHI, Toshimasa, ADACHI, Asaho, NOMIYAMA, Harumi, ISHIZAKI, Ayaaki, NAKAYAMA, Jiro, SONOMOTO, Kenji |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; January 2004, Vol. 68 Issue: 8 p1663-1671, 9p |
| Abstrakt: |
We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneriISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA(a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukMthrough ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukAand nukMgenes were enhanced by osmotic stress. The expression level of the nukAtranscript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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