| Autor: |
TAMANO, Koichi, SATOH, Yuki, ISHII, Tomoko, TERABAYASHI, Yasunobu, OHTAKI, Shinsaku, SANO, Motoaki, TAKAHASHI, Tadashi, KOYAMA, Yasuji, MIZUTANI, Osamu, ABE, Keietsu, MACHIDA, Masayuki |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; April 2007, Vol. 71 Issue: 4 p926-934, 9p |
| Abstrakt: |
The biological role of ExgA (Exg1), a secretory β-1,3-exoglucanase of Aspergillus oryzae, and the expression pattern of the exgA(exg1) gene were analyzed. The exgAdisruptant and the exgA-overexpressing mutant were constructed, and phenotypes of both mutants were compared. Higher mycelial growth rate and conidiation efficiency were observed for the exgA-overexpressing mutant than for the exgAdisruptant when β-1,3-glucan was supplied as sole carbon source. On the other hand, no difference in phenotype was observed between them in the presence or absence of the inhibitors of cell wall β-glucan remodeling when grown with glucose. exgAExpression was induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgAexpression. These data suggest that ExgA plays a role in β-glucan utilization, but is not much involved in cell wall β-glucan remodeling. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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