Exopolysaccharide Production from Sweet Potato-ShochuDistillery Wastewater by Lactobacillus SakeiCY1

Autor: Yuliani, Emma, Imai, Tsuyoshi, Teeka, Jantima, Tomita, Shintaro, Suprayogi
Zdroj: Biotechnology & Biotechnological Equipment; January 2011, Vol. 25 Issue: 2 p2329-2333, 5p
Abstrakt: ABSTRACTIn order to examine a potential use of sweet potato-shochu distillery wastewater, we investigated the production of exopolysaccharides (EPSs) by bacteria grown in wastewater-supplemented media. The supernatant of the clarified distillery by-product contains 40.5 g/L protein, 3.47 g/L nitrogen, and 32 g/L total organic carbon. Lactobacillus sakei CY1, which was obtained from cucumber fermentation, was cultured in this wastewater for exopolymer production. The highest yield of Lactobacillus sakei EPS were obtained at 4.6 g/L on basal medium supplemented with 75% shochu wastewater at 30°C and pH 6.2. Under these culture conditions, the EPSs produced comprised 25.5% glucose and 13.2% galactose, along with other sugars. The sweet potato-shochu distillery wastewater contains nutrients that support Lactobacillus sakei CY1 growth. Because the culture media required no or very little supplementation with expensive commercial nutrients, this process was not expensive.
Databáze: Supplemental Index