| Autor: |
Robb, Christopher W., Orihuela, Carlos J., Ekkelenkamp, Miquel B., Niesel, David W. |
| Zdroj: |
Gene; January 2001, Vol. 262 Issue: 1-2 p169-177, 9p |
| Abstrakt: |
Shigellagenes expressed during infection likely contribute to adaptation and virulence in the host. Using differential display PCR (DDPCR), a cDNA fragment from Shigella flexneriserotype 5 that showed enhanced expression in a murine model was identified, cloned and sequenced. Enhanced expression was verified by RNA dot blot. The full-length gene was cloned using PCR and sequenced. The complete gene sequence was BLAST searched against GenBank, and exhibited strong homology to genes encoding Haemophilus influenzaeD15 and Pasteurella multocidaOma87 protective outer membrane antigens. The S. flexnerigene putatively encodes a ∼90-kDa protein and was termed oma90. The deduced amino acid sequence from oma90was analyzed and compared to the D15/Oma87 antigens. Additionally, oma90mapped to a cluster of orthologous groups, and probably contains an ancient conserved domain. The chromosomal organization of oma90was similar to that for H. influenzaeand P. multocidaas well as for other known homologues. Northern blot revealed that the oma90transcript encoded only oma90.This report represents the first description of a S. flexnerigene identified based on enhanced expression in the host. Furthermore, we report the first evidence demonstrating in vivo regulation of a member of the d15/oma87gene family. |
| Databáze: |
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