| Abstrakt: |
The composition and the nature of the linkage of fatty acids and the Shwartzman activity of lipopolysaccharide (LPS) preparations derived from oral gram‐negative bacteria including Bacteroides gingivalis, Bacteroides loesheii, Eikenella corrodens, Fusobacterium nucleatum, and Actinobacillus actinomycetemcomitanswere examined. 3‐Hydroxylated and nonhydroxy fatty acids of various chain lengths were found in all of the LPS preparations. All nonhydroxy fatty acids were found to be ester‐bound, and part of the 3‐hydroxy fatty acids in the LPS of B. gingivalis, E. corrodens, F. nucleatum, and A. actinomycetemcomitanswere shown to be involved in ester linkage. It was also suggested that the hydroxy group of the ester‐bound 3‐hydroxy fatty acid of the LPS of F. nucleatumand A, actinomycetemcomitansis at least partly substituted by another fatty acid, but in the LPS of B. gingivalisand E. corrodensit is not. The main amide‐linked fatty acid of the LPS of B. gingivalis, E. corrodens, F. nucleatum, and A. actinomycetemcomitanswas 3‐hydroxyheptadecanoic, 3‐hydroxydodecanoic, 3‐hydroxyhexadecanoic, and 3‐hydroxytetradecanoic acid, respectively. The results of the Shwartzman assay showed that the E. corrodensLPS was the most active among the preparations tested, and that the Shwartzman toxicity of BacteroidesLPS is extremely low. |
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