| Abstrakt: |
A monoclonal antibody against the Yersinia enterocolitica60‐kilodalton (kDa) antigen, designated cross‐reacting protein antigen (CRPA), was obtained by cell fusion. The CRPA common to gram‐negative bacteria was purified from Y. enterocoliticaby the affinity chromatography with the monoclonal antibody (IgGl) thus obtained. The purified CRPA showed a single band of 60 kDa in SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE), and reacted with rabbit antisera against Y. enterocolitica, Vibrio cholerae, Escherichia coli, Pseudomonas aeruginosa, and Shigella sonneiin Western blot analysis. The monoclonal antibody, however, reacted with a 60 kDa peptide from Y. enterocolitica, but not with the antigens from other gram‐negative bacteria such as V. cholerae, E. coli, S. sonnei, Salmonella enteritidis, Serratia marcescens, Klebsiella pneumoniae, Proteus mirabilis, and P. aeruginosa. The results suggested that both species‐specific and cross‐reactive epitopes were present on a CRPA molecule. |
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