Production of a Monoclonal Antibody That Recognizes the Lipopolysaccharide of a Campylobacter‐Like Organism

Autor: Kuan, Steven Kok Chee, Coloe, Peter John, Alderton, Malcolm Raymond
Zdroj: Microbiology and Immunology; August 1992, Vol. 36 Issue: 8 p791-801, 11p
Abstrakt: A monoclonal antibody was produced to a Campylobacter‐like organism (RMIT 32A) which was isolated from the terminal ileum of a pig with proliferative enteritis. Isotyping of the antibody revealed that it was an IgG2awith kappa light chains. Immunoblots using the antibody against proteinase‐K‐treated whole cell lysates of RMIT 32A, a selection of Campylobacterspecies and other enteric bacteria showed that the antibody was specific for RMIT 32A and was directed against the lipopolysaccharide. This antibody can be used for the specific detection of RMIT 32A.
Databáze: Supplemental Index