| Autor: |
Uesaka, Yoshihiko, Otsuka, Yoko, Kashida, Mitsuaki, Oku, Yuichi, Horigome, Kazuki, Nair, G. Balakrish, Pal, S. C., Yamasaki, Shinji, Takeda, Yoshifumi |
| Zdroj: |
Microbiology and Immunology; January 1992, Vol. 36 Issue: 1 p43-53, 11p |
| Abstrakt: |
A bead‐enzyme linked immunosorbent assay (bead‐ELISA) for detection and quantification of cholera toxin (CT) in broth cultures of Vibrio choleraeO1 has been developed. Under optimal buffer and pH conditions the bead‐ELISA could consistently detect 40 pg/ml of CT. None of the ingredients of commonly used media for in vitroculture of V. choleraeO1 hindered the performance of the bead‐ELISA. Evaluation of the sensitivity and specificity of the bead‐ELISA against the commonly used reversed passive latex agglutination (RPLA) test for detection of CT was performed using a collection of 239 strains of V. choleraeO1 (including both biotypes and serotypes) which were examined by a gene probe encoding for the A1 subunit of CT. Although both the assays were highly specific, the bead‐ELISA was more sensitive than the RPLA. Quantification of CT by the bead‐ELISA revealed that the concentration of CT produced by the strains of V. choleraeO1 which were negative by the RPLA was lower than 1 ng/ml and therefore below the minimum detection ability of the RPLA. The bead‐ELISA is a simple, specific and highly sensitive assay for routine detection of CT and is recommended for routine use in clinical microbiology laboratories. |
| Databáze: |
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