| Autor: |
Saruta, Katsutoshi, Hoshina, Sadayori, Machida, Katsuhiko |
| Zdroj: |
Microbiology and Immunology; November 1995, Vol. 39 Issue: 11 p839-844, 6p |
| Abstrakt: |
Staphylococcus aureusis the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureusthrough a single‐base‐pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to detect and identify S. aureuswithout requiring additional analytical techniques. Twenty‐eight staphylococcal and non‐staphylococcal strains were tested to verify the specificity of the assay, and only S. aureusstrains gave a positive reaction. It may be possible to provide immediate and exact information for the identification of S. aureus. |
| Databáze: |
Supplemental Index |
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