| Autor: |
Nagaoka, Hiromi, Akiyama, Masato, Sugieda, Masaaki, Nishio, Tomohiro, Akahane, Sousuke, Hattori, Hiroshi, Ho, To, Fukushi, Hideto, Hirai, Katsuya |
| Zdroj: |
Microbiology and Immunology; February 1996, Vol. 40 Issue: 2 p147-151, 5p |
| Abstrakt: |
The prevalence of Coxiella burnetiiantibodies was investigated by indirect immunofluorescence (IF) test in 55 paired sera (acute and convalescent phases) of school children who had influenza‐like symptoms. Of the convalescent serum samples examined, 18 (32.7%) sera reacted positively to phase II antigen of C. burnetii. Coxiella‐like organism was isolated from the sera of 13 children after injection of the 18 acute phase sera into mice. The organism was identified as C. burnetiiby Giemsa staining and the IF antigen test of mouse spleen smears, the polymerase chain reaction (PCR) method, electron microscopic observations of the mouse spleen cells, and the IF antibody test of mouse sera. This is the first report of isolation of C. burnetiifrom serum specimens of children having influenza‐like symptoms. The evidence that C. burnetiiwas isolated from people indigenous to Japan at a considerably high incidence suggested that C. burnetiimay be widespread as a cause of influenza‐like symptoms in Japan. |
| Databáze: |
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