| Autor: |
To, Ho, Hotta, Akitoyo, Zhang, Guo Quan, Van Nguyen, Sa, Ogawa, Motohiko, Yamaguchi, Tsuyoshi, Fukushi, Hideto, Amano, Ken‐ichi, Hirai, Katsuya |
| Zdroj: |
Microbiology and Immunology; February 1998, Vol. 42 Issue: 2 p81-85, 5p |
| Abstrakt: |
Eighteen Coxiella burnetiistrains from a variety of clinical and geographical sources were screened for antigenic variation of polypeptides by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) coupled with Coomassie brilliant blue (CBB) staining or immunoblotting. These polypeptide profiles showed the greatest variability in the region from 33 to 8.1 kDa. Such differences in the antigenicity of the polypeptides were also recognized by immunoblotting with 15 various mouse anti‐C. burnetiiantisera. In addition, we detected a polypeptide at about 28 kDa which was immunodominant in strains from human cases of acute Q fever, milk and ticks but not immunogenic in strains from human cases of chronic Q fever. These findings suggest that this polypeptide is a marker to distinguish between acute and chronic strains. |
| Databáze: |
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