| Autor: |
Lyoda, Sunao, Wada, Akihito, Weller, Jeniffer, Flood, Susan J. A., Schreiber, Edgar, Tucker, Bill, Watanabe, Haruo |
| Zdroj: |
Microbiology and Immunology; August 1999, Vol. 43 Issue: 8 p803-806, 4p |
| Abstrakt: |
The amplified fragment‐length polymorphism (AFLPTM) technique is based on the selective PCR amplification of restriction fragments. We investigated the utility of AFLP in the molecular subtyping of enterohemorrhagic Escherichia coliserotype O157:H7 isolates. We analyzed a total of 46 isolates of E. coliO157:H7 along with other serotypes, O26:H11, O114:H19 and O119:NT. Isolates of E. coliO157:H7 derived from the same outbreak showed an identical AFLP‐banding pattern and were subtyped into the same group, giving results almost consistent with those of a pulsed‐field gel electrophoresis (PFGE) study, while other serotypes showed clearly different patterns from those of E. coliO157:H7. These results suggest that the AFLP technique has potential as an alternative tool for the molecular epidemiology of E. coliO157:H7. |
| Databáze: |
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