| Autor: |
Egea, Amandine, Metivier, Melinda, Croguennoc, Philippe, Remaud-Simeon, Magali, Vieu, Christophe |
| Zdroj: |
BioNanoScience; March 2014, Vol. 4 Issue: 1 p37-45, 9p |
| Abstrakt: |
In this work, a dynamic enzymatic reaction based on dextransucrase is monitored in real-time and in liquid environment through Surface-Enhanced Ellipsometric Contrast microscopy. This noninvasive technique allows in situ observations with high lateral spatial resolution and extreme vertical sensitivity, due to the particular surfaces involved. These surfaces, called Surfs, are the core of Surface-Enhanced Ellipsometric Contrast microscopy. They drastically enhance the sample contrast and enable direct visualization of nanometer thick films down to 0.3 nm. Therefore, in situ observations of biomolecules immobilized on a surface become possible in their physiological environment. In this perspective, microcontact printing is used to deposit dextransucrase enzymatic patterns on a thin layer of PLL-g-dextran and the dextransucrase polymerization is characterized for 120 min in biocompatible conditions. Quantitative data of the polymer growth on these microscale patterns are obtained with nanometric resolution in thickness. This study opens new paths for understanding the dynamic behavior of confined molecules such as the ones involved in bioengineered surfaces and could be applied for monitoring reactions kinetics. |
| Databáze: |
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