Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon × neuroblastoma hybrid cell line MES23.5

Autor: Schneider, T., Perez-Reyes, E., Nyormoi, O., Wei, X., Crawford, G.D., Smith, R.G., Appel, S.H., Birnbaumer, L.
Zdroj: Neuroscience; 1995, Vol. 68 Issue: 2 p479-485, 7p
Abstrakt: The identity of α1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon × N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(α1D, Q-(αA) and ω-contoxin GVIA sensitive N-type (α1B) Ca2+ channel α1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known α1 subunits, reveal splice variation in the L- and Q-type α1 subunit of MES23.5 cells. The transcripts of α1 subunits in these cells were quantified by RNAase protection assay.
Databáze: Supplemental Index