| Autor: |
Rossman, Jon E., Caty, Michael G., Rich, Gary A., Karamanoukian, Hratch L., Azizkhan, Richard G. |
| Zdroj: |
Journal of Pediatric Surgery; August 1996, Vol. 31 Issue: 8 p1147-1151, 5p |
| Abstrakt: |
The in vitro effects of perfluorocarbon (PFC) on human neutrophil activation and chemotaxis were examined. Neutrophils were incubated, with and without PFC, and were analyzed for chemotaxis through 5-μm and 8-μm pore filters. Neutrophil activation was quantitated by flow cytometry. Activation studies showed that PFC-treated neutrophil samples (n = 6) produced only 39.83% ± 25.9% (mean ± SD) of matched control (n = 6) fluorescence. Chemotaxis studies showed that PFC-treated neutrophil samples (n = 8) had migration of only 18.63% ± 6.5% (of control values) of neutrophils to the outer boundary of the 5-μm filters (n = 8 controls). The 8-μm pore filter migration (n = 8) was similarly low, with a mean outer boundary migration count of only 26% ± 19.8% of the control (n =8) value. Thus, neutrophils exposed to perfluorocarbon produce significantly less detectable H2O2(P< .001) and have a significantly lower chemotactic response (P < .001). |
| Databáze: |
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