| Autor: |
Antonio, D.B., Andree, K.B., McDowell, T.S., Hedrick, R.P. |
| Zdroj: |
Journal of Aquatic Animal Health; December 1998, Vol. 10 Issue: 4 p338-347, 10p |
| Abstrakt: |
AbstractA nonradioactive in situ hybridization (ISH) protocol was developed to detect Myxobolus cerebralis, the causative organism of whirling disease, in its primary host, rainbow trout Oncorhynchus mykiss, and in its alternate oligochaete host, Tubifex tubifex.A cocktail of three oligonucleotide primers (derived from the small subunit ribosomal DNA sequence) directed at target sequences of the parasite DNA was tailed at the 3′ end with digoxigenin-labeled deoxyuridine triphosphate (DIG-dUTP). Labeled probes were hybridized to parasite DNA present in deparaffinized tissue sections from infected trout and oligochaetes. The bound probes were visualized after modifications of existing ISH protocols. By using the new ISH procedure, the parasite was found in target tissues of subclinically and clinically infected fish and tubificid oligochaetes after exposures of these hosts to triactinomyxons and mature spores, respectively. The probe did not bind with salmonid tissues infected with two other myxosporean parasites, Ceratomyxa shastaor the PKX organism, or to a Myxobolussp. infecting the cartilage of plain sculpin Myoxocephalus jaok.These initial results indicate that ISH is an effective and specific test for detecting Myxobolus cerebralisin its fish and oligochaete hosts. |
| Databáze: |
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