| Autor: |
Burreson, E.M., Stokes, N.A., Friedman, C.S. |
| Zdroj: |
Journal of Aquatic Animal Health; March 2000, Vol. 12 Issue: 1 p1-8, 8p |
| Abstrakt: |
AbstractThe protistan parasite Haplosporidium nelsonihas caused extensive mortality in the eastern oyster Crassostrea virginicaalong the mid-Atlantic coast of the United States since 1957. The origin of H. nelsonihas remained unresolved. Molecular diagnostic tools were used to examine the hypothesis that a haplosporidian parasite in the Pacific oyster C. gigasis H. nelsoni.A DNA probe specific for H. nelsonireacted positively in in situ hybridizations with haplosporidian plasmodia from C. gigascollected in Korea, Japan, and California. Primers that specifically amplify H. nelsoniDNA in the polymerase chain reaction amplified product from Californian C. gigasinfected with the haplosporidian parasite. The DNA sequence of the 565-base pair amplified product was identical to the H. nelsonisequence except for a single nucleotide transition, a similarity of 99.8%. These results are conclusive evidence that the parasite in C. gigasis H. nelsoniand strongly support previous speculation that the parasite was introduced into Californian populations of C. gigasfrom Japan. Results also support previous speculation that H. nelsoniwas introduced from the Pacific Ocean to C. virginicaon the East Coast of the United States, likely with known importations of C. gigas.These results document greatly increased virulence in a naive host–parasite association and reinforce potential dangers of intentional, but improper, introductions of exotic marine organisms for aquaculture or resource restoration. |
| Databáze: |
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