| Autor: |
Mathison, Bridget D., Kimble, Lindsey L., Kaspar, Kerrie L., Khoo, Christina, Chew, Boon P. |
| Zdroj: |
Journal of Natural Products; September 2013, Vol. 76 Issue: 9 p1605-1611, 7p |
| Abstrakt: |
Vaccinium macrocarpon(cranberry) products have been used to prevent uropathogenic Escherichia(E.) coliadherence to uroepithelial cells (UEC) and may help reduce risk of urinary tract infection. Reported herein are the development and validation of an assay to assess antiadhesion activity of V. macrocarponextracts and human urine. P-fimbriated E. coli(CFT073) was labeled with 3H-uridine, then co-incubated with HTB-4 UEC at a 400:1 ratio. V. macrocarponextracts (0–17 mg proanthocyanidins/mL) were added to 3H-labeled E. colibefore co-incubating with UEC. The assay yielded a sensitive inhibition curve: the lower limit of detection and half-maximal inhibitory concentration were 0.43 and 1.59 mg proanthocyanidins/mL for V. macrocarponextract CEP 55; intra- and interassay coefficients of variance were <10% and <15%, respectively. V. macrocarponextract CEP 3283 showed identical adhesion inhibition. Serial dilutions of urine from human participants who consumed V. macrocarponbeverages showed a linear decrease in antiadhesion activity. Antiadhesion assays conducted with urine from a human intervention study also showed good agreement with results obtained using the hemagglutination assay. Therefore, a sensitive, high-throughput, biologically relevant antiadhesion assay using 3H-E. colico-incubated with UEC is reported, which can be used for studying the action of V. macrocarponbioactives. |
| Databáze: |
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