| Autor: |
ElSohly, Mahmoud A., Little, Thomas L., Stanford, Donald F. |
| Zdroj: |
Journal of Analytical Toxicology; May 1992, Vol. 16 Issue: 3 p188-188, 1p |
| Abstrakt: |
GC/MS analysis of biological specimens is believed to be the most forensically accepted method for confirming the presence of abused drugs. 11-Nor-Δ9-tetrahydrocannabinol- 9-carboxylic acid (Δ9-THC-COOH) is the major metabolite of Δ9-tetrahydrocannabinol (Δ9-THC) for which testing (including GC/MS) is directed as an indication of marijuana use. The currently available internal standard for Δ9-THC-COOH is d3-Δ9-THC-COOH, which has the deuterium atoms located on the side chain. In addition to the high cost of this compound, it suffers from a limited dynamic range of analysis, especially when the methyl derivative is used. This is because of a contribution to one of the internal standard ions (m/z 316) from a fragmentation of the natural drug which involves loss of the side chain. The new internal standard, d6-11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (d6-Δ9-THC-COOH), avoids these disadvantages. The six deuterium atoms are located on the two methyl groups of Carbon 6 in the dibenzopyran structure. The dynamic range of analysis with the new internal standard was tested between 6.25 to 1,000 ng/mL with a correlation coefficient of 0.998. Analysis of several urine specimens for Δ9-THC metabolite using both d3- and d6- internal standards showed a correlation coefficient of 0.9987. |
| Databáze: |
Supplemental Index |
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