| Autor: |
Wallace, Richard J., Iakhiaeva, Elena, Williams, Myra D., Brown-Elliott, Barbara A., Vasireddy, Sruthi, Vasireddy, Ravikiran, Lande, Leah, Peterson, Donald D., Sawicki, Janet, Kwait, Rebecca, Tichenor, Wellington S., Turenne, Christine, Falkinham, Joseph O. |
| Zdroj: |
Journal of Clinical Microbiology; June 2013, Vol. 51 Issue: 6 p1747-1752, 6p |
| Abstrakt: |
ABSTRACTRecent studies have shown that respiratory isolates from pulmonary disease patients and household water/biofilm isolates of Mycobacterium aviumcould be matched by DNA fingerprinting. To determine if this is true for Mycobacterium intracellulare, household water sources for 36 patients with Mycobacterium aviumcomplex (MAC) lung disease were evaluated. MAC household water isolates from three published studies that included 37 additional MAC respiratory disease patients were also evaluated. Species identification was done initially using nonsequencing methods with confirmation by internal transcribed spacer (ITS) and/or partial 16S rRNA gene sequencing. M. intracellularewas identified by nonsequencing methods in 54 respiratory cultures and 41 household water/biofilm samples. By ITS sequencing, 49 (90.7%) respiratory isolates were M. intracellulareand 4 (7.4%) were Mycobacterium chimaera. In contrast, 30 (73%) household water samples were M. chimaera, 8 (20%) were other MAC X species (i.e., isolates positive with a MAC probe but negative with species-specific M. aviumand M. intracellulareprobes), and 3 (7%) were M. avium; none were M. intracellulare. In comparison, M. aviumwas recovered from 141 water/biofilm samples. These results indicate that M. intracellularelung disease in the United States is acquired from environmental sources other than household water. Nonsequencing methods for identification of nontuberculous mycobacteria (including those of the MAC) might fail to distinguish closely related species (such as M. intracellulareand M. chimaera). This is the first report of M. chimaerarecovery from household water. The study underscores the importance of taxonomy and distinguishing the many species and subspecies of the MAC. |
| Databáze: |
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