| Autor: |
Alam, Mohammad Murshid, Tsai, Lillian L., Rollins, Sean M., Sheikh, Alaullah, Khanam, Farhana, Bufano, Meagan Kelly, Yu, Yanan, Wu-Freeman, Ying, Kalsy, Anuj, Sultana, Tania, Sayeed, M. Abu, Jahan, Nusrat, LaRocque, Regina C., Harris, Jason B., Leung, Daniel T., Brooks, W. Abdullah, Calderwood, Stephen B., Charles, Richelle C., Qadri, Firdausi, Ryan, Edward T. |
| Zdroj: |
Clinical and Vaccine Immunology (formerly CDLI); March 2013, Vol. 20 Issue: 5 p712-719, 8p |
| Abstrakt: |
ABSTRACTSalmonella entericaserotype Paratyphi A is a human-restricted pathogen and the cause of paratyphoid A fever. Using a high-throughput immunoscreening technique, in vivo-induced antigen technology (IVIAT), we identified 20 immunogenic bacterial proteins expressed in humans who were bacteremic with S. Paratyphi A but not those expressed in S. Paratyphi A grown under standard laboratory conditions. The majority of these proteins have known or potential roles in the pathogenesis of S. enterica. These include proteins implicated in cell adhesion, fimbrial structure, adaptation to atypical conditions, oxidoreductase activity, proteolysis, antimicrobial resistance, and ion transport. Of particular interest among these in vivo-expressed proteins were S. Paratyphi A (SPA)2397, SPA2612, and SPA1604. SPA2397 and SPA2612 are prophage related, and SPA1604 is in Salmonellapathogenicity island 11 (SPI-11). Using real-time quantitative PCR (RT-qPCR), we confirmed increased levels of mRNA expressed by genes identified by IVIAT in a comparison of mRNA levels in organisms in the blood of bacteremic patients to those in in vitrocultures. Comparing convalescent- to acute-phase samples, we also detected a significant increase in the reaction of convalescent-phase antibodies with two proteins identified by IVIAT: SPA2397 and SPA0489. SPA2397 is a phage-related lysozyme, Gp19, and SPA0489 encodes a protein containing NlpC/P60 and cysteine, histidine-dependent amidohydrolase/peptidase (CHAP) domains. In a previous study utilizing a different approach, we found that transcripts for 11 and 7 of the genes identified by IVIAT were detectable in organisms in the blood of humans in Bangladesh who were bacteremic with S. Paratyphi A and Salmonella entericaserovar Typhi, respectively. S.Paratyphi A antigens identified by IVIAT warrant further evaluation for their contributions to pathogenesis and might have diagnostic, therapeutic, or preventive relevance. |
| Databáze: |
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