| Autor: |
Nagamitsu, Hiroshi, Murata, Masayuki, Kosaka, Tomoyuki, Kawaguchi, Junpei, Mori, Hirotada, Yamada, Mamoru |
| Zdroj: |
Journal of Molecular Microbiology and Biotechnology; 20240101, Issue: Preprints p227-232, 6p |
| Abstrakt: |
σE-dependent cell lysis has been proposed to eliminate damaged cells in the stationary phase in Escherichia coli. In order to explore the relationship of this process to long-term stationary phase existence, we considered that micA and rybB could be important small regulatory RNA (sRNA) genes for σE-dependent cell lysis. A long-term stationary phase was observed at temperatures of <37°C, but not >38°C, and was found even in an rpoS knock-out background. Strains with disrupted micA or rybB were incapable of long-term stationary phase existence. Both strains drastically lost survivability accompanied by a dramatic accumulation of mutations. These findings allow us to speculate that σE-dependent cell lysis plays a key role in the establishment of the long-term stationary phase, presumably by eliminating damaged cells and thus preventing the over-accumulation of mutations. |
| Databáze: |
Supplemental Index |
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