| Autor: |
van Rensburg, B. Janse, Flett, B.C., Mc Laren, N.W., Mc Donald, A.H. |
| Zdroj: |
South African Journal of Plant and Soil; January 2008, Vol. 25 Issue: 2 p105-110, 6p |
| Abstrakt: |
Fusariumspp. have variable phenotypic and morphological features when cultured on different media. Inaccurate identifications could result in variation in the quantification of Fusariumspp. associated with ear rot of maize when two or more researchers identify the same Fusariumisolates based on morphological characteristics. Two hundred and fifty kernels from maize samples collected from each of five localities were surface-sterilized and plated on selective rose Bengal-glycerine-urea (RbGU) medium from which isolates were subsequently transferred to split plates containing Carnation Leaf Agar (CLA) and Potato Dextrose Agar (PDA) for further identification. Three different enumerators independently identified and enumerated Fusariumspp. on RbGU and CLA/ PDA media to determine the relative frequency of Fusariumspp. within maize samples, to compare the accuracy of species identification on different media and to detect and quantify bias among enumerators (inter-enumerator reliability). It was concluded that enumerators were consistent in the identification and quantification of F. verticillioidesand F. subglutinanson both RbGU and CLA/PDA media. F. proliferatumenumerations yielded an interaction between locality and enumerator with no significant differences for media. The significant locality x enumerator interaction for F. proliferatumsuggests that enumerators had difficulty to distinguish between F. proliferatumand F. verticillioides. The use of RbGU and/or CLA/PDA media were eliminated as a possible source of variation in the identification of Fusariumspp. The inter-enumerator reliability study indicated that Fusariumspp. enumerations by three different enumerators were reliable and accurate and were not a source of variation in the quantification of Fusariumspp. associated with maize ear rot. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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