| Autor: |
Carneiro, Ana Carolina Aguiar Vasconcelos, Andrade, Gláucia Manzan, Costa, Júlia Gatti Ladeia, Pinheiro, Breno Veloso, Vasconcelos-Santos, Daniel Vitor, Ferreira, Adriana Melo, Su, Chunlei, Januário, José Nélio, Vitor, Ricardo Wagner Almeida |
| Zdroj: |
Journal of Clinical Microbiology; March 2013, Vol. 51 Issue: 3 p901-907, 7p |
| Abstrakt: |
ABSTRACTRecent studies of Toxoplasma gondiiisolates from animals in Brazil have revealed high genetic diversity. Many of these isolates are virulent to mice. It is speculated that these isolates may also be virulent to humans. However, there is very limited data regarding T. gondiistrains from human infection. Therefore, it is not clear whether there is any association between parasite genotypes and disease phenotypes. In this study, a total of 27 T. gondiistrains were isolated from humans with congenital toxoplasmosis in Minas Gerais state, Brazil. The genetic variability was assessed by restricted fragment length polymorphism in 11 loci (SAG1, 5' plus 3' SAG2, alternative [alt.] SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). Genetic analysis of 24 strains revealed 14 different genotypes, including 7 previously identified from animals and 7 new types. The widespread genotype BrII accounted for 29% (7/24) of the isolates and was the dominant genotype involved in this study. This is the first report of genotyping of T. gondiiisolates obtained from blood samples from newborns with congenital toxoplasmosis. Genotypic characterization of these isolates suggests high genetic diversity of T. gondiiin this human population in Brazil. Future studies are needed to determine the source of contamination of this human population. |
| Databáze: |
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