| Autor: |
Tadesse, Daniel A., Bahnson, Peter B., Funk, Julie A., Morrow, W.E. Morgan, Abley, Melanie J., Ponte, Valeria A., Thakur, Siddhartha, Wittum, Thomas, DeGraves, Fred J., Rajala-Schultz, Paivi J., Gebreyes, Wondwossen A. |
| Zdroj: |
Foodborne Pathogens & Disease; January 2013, Vol. 10 Issue: 1 p80-86, 7p |
| Abstrakt: |
AbstractYersinia enterocoliticais an important foodborne pathogen, and pigs are recognized as a major reservoir and potential source of pathogenic strains to humans. A total of 172 Y. enterocoliticarecovered from conventional and antimicrobial-free pig production systems from different geographic regions (North Carolina, Ohio, Michigan, Wisconsin, and Iowa) were investigated to determine their pathogenic significance to humans. Phenotypic and genotypic diversity of the isolates was assessed using antibiogram, serogrouping, and amplified fragment length polymorphism (AFLP). Carriage of chromosomal and plasmid-borne virulence genes were investigated using polymerase chain reaction. A total of 12 antimicrobial resistance patterns were identified. More than two-thirds (67.4%) of Y. enterocoliticawere pan-susceptible, and 27.9% were resistant against β-lactams. The most predominant serogroup was O:3 (43%), followed by O:5 (25.6%) and O:9 (4.1%). Twenty-two of 172 (12.8%) isolates were found to carry Yersiniaadhesion A (yadA), a virulence gene encoded on the Yersiniavirulence plasmid. Sixty-nine (40.1%) isolates were found to carry ailgene. The ystAand ystBgenes were detected in 77% and 26.2% of the strains, respectively. AFLP genotyping of isolates showed wide genotypic diversity and were grouped into nine clades with an overall genotypic similarity of 66.8–99.3%. AFLP analysis revealed that isolates from the same production system showed clonal relatedness, while more than one genotype of Y. enterocoliticacirculates within a farm. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
