| Autor: |
Torina, Alessandra, Agnone, Annalisa, Blanda, Valeria, Alongi, Angelina, D’Agostino, Rosalia, Caracappa, Santo, Marino, Anna M.F., Di Marco, Vincenzo, de la Fuente, José |
| Zdroj: |
Ticks and Tick-borne Diseases; December 2012, Vol. 3 Issue: 5-6 p283-287, 5p |
| Abstrakt: |
Anaplasma ovisand Anaplasma marginaleare tick-transmitted bacteria that cause anaplasmosis in domestic and wild animals. Recent results show that some domestic and wild animals and ticks are susceptible to both A. ovisand A. marginale, thus supporting the need to differentiate between these species in hosts and ticks diagnosed with Anaplasmainfection. However, although anaplasmosis is one of the most common diseases of grazing animals worldwide, rapid and effective tests are not available for the detection of and discrimination between these 2 Anaplasmaspecies. The objective of this research was to develop an easy and reliable method to identify and discriminate between the closely related pathogens A. ovisand A. marginale. A. ovisand A. marginalemajor surface protein 4 (msp4) gene sequences were retrieved from different geographic strains and aligned to design 2 sets of primers in a region with significant differences between the 2 species, but completely conserved among strains. PCR reactions using these primers were 100% species-specific and detected all strains from each pathogen previously identified with other methods. The 2 sets of primers designed for the specific PCR amplification of A. ovisand A. marginaleallow easy-to-detect and discriminate between the 2 pathogens, thus avoiding the time-consuming sequencing or multi-gene amplification procedures. This PCR provides a tool for the detection of A. ovisand A. marginalein ticks and in wildlife and domestic hosts. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect