| Autor: |
Duve, Hanne, Johnsen, Anders H., Maestro, Jose-Luis, Scott, Alan G., East, Peter D., Thorpe, Alan |
| Zdroj: |
Regulatory Peptides; November 1996, Vol. 67 Issue: 1 p11-19, 9p |
| Abstrakt: |
Information from the Leu-callatostatin gene sequences of the blowflies Calliphora vomitoriaand Lucilia cuprinawas used to develop antisera specific for the variable post-tyrosyl amino-acid residues Ser, Ala and Asn of the common Leu-callatostatin C-terminal pentapeptide sequence −YXFGL-NH2. Radioimmunoassays based on these antisera were used to purify peptides from an extract of 40 000 blowfly heads. Five neuropeptides of the Leu-callatostatin family were identified. Three have a seryl residue in the post-tyrosyl position. Two of these are octapeptides that differ only at the N-terminal residue; NRPYSFGL-NH2and ARPYSFGL-NH2, whilst the third is the heptapeptide derived by N-terminal trimming; RPYSFGL-NH2. Two octapeptides in which X is Ala and Asn were also identified; VERYAFGL-NH2and LPVYNFGL-NH2. The latter peptide is derived by processing at the internal dibasic site of a putative heneicosapeptide encoded by the DNA. These findings stress the necessity to have putative structures verified at the peptide level. Potent, reversible inhibitory effects on the spontaneous contractile activity of the blowfly rectum were recorded for ARPYSFGL-NH2(monophasic dose-response curve with an IC50= 10 fM) and for LPVYNFGL-NH2(biphasic dose-response curve with IC50values of approximately 1 fM and 1 nM). It is suggested that regulation of gut motility in insects, rather than an allatostatic function, may represent an ancestral and universal function of the allatostatins. One of the reasons for the large number of members of the Leu-callatostatin family appears to be in the provision of an integrated form of gut motility control, with different peptides controlling specific regions of the gut. |
| Databáze: |
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