QUANTITATIVE ESTIMATION OF TOTAL PROTEIN OF CEREBROSPINAL FLUID: A Modification and Improvement of a Previously Described Method

Autor: Young, G. Alexander, Bennett, A. E., Christlieb, J. M., Myers, John T.
Zdroj: Archives of Neurology and Psychiatry; March 1930, Vol. 23 Issue: 3 p542-545, 4p
Abstrakt: In a previous report,1 we described a new practical method of quantitative total protein estimation in cerebrospinal fluid. We wish to correct some errors in this method that have been learned by further investigation and also to describe a modification of the method. This modification has proved to be a more accurate and simpler technic than that previously described.The original method was by precipitation of protein in cerebrospinal fluid by 95 per cent alcohol, acetic acid and heat. The resultant precipitate was centrifugated in a capillary-tipped vaccine tube and the total amount of protein measured in hundredths of a cubic centimeter. This method has proved of practical clinical value and a definite improvement over the qualitative tests for globulin.In working with other protein precipitants we have found that the original method was not a total protein method, but probably a total globulin precipitation, because when other
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