| Autor: |
Zhou, Jing C., Chuang, Maria H., Lan, Esther H., Dunn, Bruce, Gillman, Patricia L., Smith, Scott M. |
| Zdroj: |
Journal of Materials Chemistry; 2004, Vol. 14 Issue: 14 p2311-2316, 6p |
| Abstrakt: |
Anti-cortisol was encapsulated in optically transparent sol–gel silica matrices and competitive immunoassays for cortisol were conducted using the antibody-doped silica material as sensing elements. Calibration curves were derived in the physiological relevant range for cortisol of 1 to 100 µg dl−1. Sensing elements were prepared in both monolith and thin-film forms, and results show that with thin films, accessibility of antigen to the encapsulated antibody was improved leading to a significant reduction in the required assay time. Moreover, non-specific binding to the sol–gel silica matrix was well controlled. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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