| Autor: |
Profita, Mirella, Sala, Angelo, Riccobono, Loredana, Paternò, Alessandra, Mirabella, Angela, Bonanno, Anna, Guerrera, Daniela, Pace, Elisabetta, Bonsignore, Giovanni, Bousquet, Jean, Vignola, Antonio M. |
| Zdroj: |
The Journal of Allergy and Clinical Immunology; April 2000, Vol. 105 Issue: 4 p711-716, 6p |
| Abstrakt: |
Background:Recent evidence shows that 15(S)-hydroxy-eicoisatetraenoic acid (15[S]-HETE) can be released and rapidly reincorporated into cellular lipids. These mechanisms exert several immunoregulatory functions that may be relevant in airway inflammation. Objective:Our purpose was to evaluate the levels of both soluble and cell-associated 15(S)-HETE and to examine 15-lipoxygenase (15-LO) messenger RNA (mRNA) expression in sputum samples obtained from 10 control and 18 asthmatic subjects. Methods:Levels of 15(S)-HETE were measured by reverse-phase HPLC separation followed by RIA in supernatants and in cell membrane–extracted phospholipids after acid hydrolysis. 15-LO mRNA was evaluated by primed in situ hybridization (PRINS). Combined immunocytochemistry and PRINS was used to identify the phenotype of cells bearing 15-LO transcripts. Results:Levels of both soluble and cell-associated 15(S)-HETE were higher in asthmatic than in control subjects (P< .0001). The percentage of cells expressing 15-LO mRNA was higher in asthmatic than in control subjects (P< .01). On double staining for specific cell-type markers and 15-LO mRNA, macrophages were the major source for 15-LO. Conclusion:This study shows that the induced sputum technique allows the evaluation of 15-LO activity and that soluble, cell-associated 15(S)-HETE and 15-LO levels are higher in asthmatic than in control subjects. In addition, this study indicates that, in induced sputum, airway macrophages are the major source of 15(S)-HETE in asthma. (J Allergy Clin Immunol 2000;105:711-6.) |
| Databáze: |
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