Clinical Evaluation of a Lysis-Centrifugation Technique for the Detection of Septicemia

Autor: Kelly, Michael T., Fojtasek, Marvin F., Abbott, Thomas M., Hale, DeVon C., Dizikes, JoAnn R., Boshard, Rebecca, Buck, George E., Martin, William J., Matsen, John M.
Zdroj: JAMA: Journal of the American Medical Association; October 1983, Vol. 250 Issue: 16 p2185-2188, 4p
Abstrakt: A commercially available lysis-centrifugation blood culture system was compared with a two-bottle broth-culture system employing 100 mL of broth and 10 mL of blood per bottle to analyze 1,913 blood specimens. Of 154 clinically significant isolates, 89% were detected by the lysis-centrifugation technique, and 73% were detected by the broth-culture method. Twenty-seven percent of the organisms were detected only by the lysis-centrifugation technique, and 11% were detected only by the broth system. Fifteen polymicrobial cultures were encountered; the lysis-centrifugation technique detected 93% of the organisms in these cultures, while the broth-culture method detected only 20%. Isolated colonies of clinically important organisms were available 30 hours earlier with the lysis-centrifugation technique. These results suggest that the lysis-centrifugation technique may provide a substantial improvement over conventional methods for blood cultures.(JAMA 1983;250:2185-2188)
Databáze: Supplemental Index