| Autor: |
Ribi, Edgar, Filz, Charles J., Goode, Granville, Strain, S. Michael, Yamamoto, Ken-ichi, Harris, Steven C., Simmons, James H. |
| Zdroj: |
Journal of Chromatographic Science; October 1970, Vol. 8 Issue: 10 p577-577, 1p |
| Abstrakt: |
A chromatographic technique for separating steroid hormones utilizes centrifugal force to enhance the migration of a sample through columns of densely packed gel. Application of centrifugal force permits the use of ultra fine silica (150 A-wide leaflets) for preparing the adsorbent bed, thereby providing improved resolution. By the use of several solvent systems various closely related species of steroids were separated, including 1) 17-keto steroids, 2) axial-equatorial hydroxyl isomers and cis-trans isomers of pregnanediol, and 3) other combinations of steroids heretofore not readily resolved, such as cortisone and prednisone, 5β-pregnane-3,20-dione and 5α-pregnane-3,20-dione, and 17α-OH progesterone and testosterone. The method is distinctive in its simplicity, high resolving power, reproducibility, rapidity, and low cost. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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