Rapid Screening of Natural Products by High-Resolution High-Temperature Gas Chromatography

Autor: Carbonell, Silvia Albero, de Aquino Neto, Francisco Radler, Cardoso, Jari Nobrega, dos Santos Pereira, Alberto, Amaral, Ana Claudia F., Barnes, Roderick A.
Zdroj: Journal of Chromatographic Science; June 2000, Vol. 38 Issue: 6 p234-234, 1p
Abstrakt: The crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus plants are fractionated by thin-layer chromatography, yielding five fractions. The fractions and the crude extract are analyzed by high-temperature high-resolution gas chromatography coupled with mass spectrometry (HT-HRGC-MS). Several natural products, including thermolabile components, can be characterized directly in the samples, such as alkaloids, terpenes, flavonoids, acids, alcohols, etc. The cold on-column technique proves to be appropriate for the injection of these thermolabile compounds. HT-HRGC-MS is shown to be a valuable tool and an alternative technique to classical phytochemical procedures for the simple and fast routine analysis of natural products in crude extracts.
Databáze: Supplemental Index