Calculation of Solute Retention for pH Gradient Elution HPLC Experiments Using Single and Coupled Columns

Autor: Buck, Christopher F., Tomellini, Sterling A.
Zdroj: Journal of Chromatographic Science; April 1989, Vol. 27 Issue: 4 p166-166, 1p
Abstrakt: An approach for calculating the retention of solutes for reversed-phase high-performance liquid chromatography involving pH gradient elution is developed and tested. The approach is an extension of previous work based on computer-assisted numerical integration rather than exact solutions for calculating solute retention. The results obtained from single- and coupled-column gradient elution experiments are comparable to those obtained using typical reversed-phase conditions.
Databáze: Supplemental Index